Respiratory Medicine
Volume 101, Issue 10 , Pages 2108-2112 , October 2007

Neutrophils in induced sputum from healthy children: Role of interleukin-8 and oxidative stress

  • Neeta Kulkarni

      Affiliations

    • Department of Infection, Immunity and Inflammation, University of Leicester, Leicester LE2 7LX, UK
    • ,
  • Marcus S. Cooke

      Affiliations

    • Department of Cancer Studies and Department of Genetics, Radiation and Oxidative Stress Section, University of Leicester, Leicester LE2 7LX, UK
    • ,
  • Jonathan Grigg

      Affiliations

    • Institute of Cell and Molecular Science, Barts and The London, Queen Mary's School of Medicine and Dentistry, 4 Newark Street, London E1 2AT, UK
    • Corresponding Author InformationCorresponding author.

Received 15 January 2007 ,Accepted 25 May 2007.

References 

  1. Gibson PG, Henry RL, Thomas P. Noninvasive assessment of airway inflammation in children: induced sputum, exhaled nitric oxide, and breath condensate. Eur Respir J. 2000;16:1008–1015
  2. Cai Y, Carty K, Henry RL, Gibson PG. Persistence of sputum eosinophilia in children with controlled asthma when compared with healthy children. Eur Respir J. 1998;11:848–853
  3. Zihlif N, Paraskakis E, Tripoli C, Lex C, Bush A. Markers of airway inflammation in primary ciliary dyskinesia studied using exhaled breath condensate. Pediatr Pulmonol. 2006;41:509–514
  4. Gibson PG, Simpson JL, Hankin R, Powell H, Henry RL. Relationship between induced sputum eosinophils and the clinical pattern of childhood asthma. Thorax. 2003;58:116–121
  5. Thomas RA, Green RH, Brightling CE, et al. The influence of age on induced sputum differential cell counts in normal subjects. Chest. 2004;126:1811–1814
  6. Liu L, Mul FP, Kuijpers TW, et al. Neutrophil transmigration across monolayers of endothelial cells and airway epithelial cells is regulated by different mechanisms. Ann NY Acad Sci. 1996;796:21–29
  7. Gibson PG, Simpson JL, Saltos N. Heterogeneity of airway inflammation in persistent asthma: evidence of neutrophilic inflammation and increased sputum Interleukin-8. Chest. 2001;119:1329–1336
  8. Jatakanon A, Lalloo UG, Lim S, Chung KF, Barnes PJ. Increased neutrophils and cytokines, TNF-alpha and IL-8, in induced sputum of non-asthmatic patients with chronic dry cough. Thorax. 1999;54:234–237
  9. Sagel SD, Kapsner R, Osberg I, Sontag MK, Accurso FJ. Airway inflammation in children with cystic fibrosis and healthy children assessed by sputum induction. Am J Respir Crit Care Med. 2001;164:1425–1431
  10. Drost EM, Skwarski KM, Sauleda J, et al. Oxidative stress and airway inflammation in severe exacerbations of COPD. Thorax. 2005;60:293–300
  11. Rahman I, MacNee W. Regulation of redox glutathione levels and gene transcription in lung inflammation: therapeutic approaches. Free Radic Biol Med. 2000;28:1405–1420
  12. Cooke MS, Evans MD, Herbert KE, Lunec J. Urinary 8-oxo-2′-deoxyguanosine—source, significance and supplements. Free Radic Res. 2000;32:381–397
  13. Kulkarni N, Pierse N, Rushton L, Grigg J. Carbon in airway macrophages and lung function in children. N Engl J Med. 2006;355:21–30
  14. Cataldo D, Foidart JM, Lau L, et al. Induced sputum: comparison between isotonic and hypertonic saline solution inhalation in patients with asthma. Chest. 2001;120:1815–1821
  15. Brightling CE, Ward R, Woltmann G, et al. Induced sputum inflammatory mediator concentrations in eosinophilic bronchitis and asthma. Am J Respir Crit Care Med. 2000;162:878–882
  16. Brightling CE, Monteiro W, Ward R, et al. Sputum eosinophilia and short-term response to prednisolone in chronic obstructive pulmonary disease: a randomised controlled trial. Lancet. 2000;356:1480–1485
  17. Bland JM, Altman DG. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet. 1986;1:307–310
  18. Geiser T, Dewald B, Ehrengruber MU, Clark-Lewis I, Baggiolini M. The interleukin-8-related chemotactic cytokines GRO alpha, GRO beta, and GRO gamma activate human neutrophil and basophil leukocytes. J Biol Chem. 1993;268:15419–15424
  19. Norzila MZ, Fakes K, Henry RL, Simpson J, Gibson PG. Interleukin-8 secretion and neutrophil recruitment accompanies induced sputum eosinophil activation in children with acute asthma. Am J Respir Crit Care Med. 2000;161:769–774
  20. Malerba M, Ricciardolo F, Radaeli A, et al. Neutrophilic inflammation and IL-8 levels in induced sputum of alpha-1-antitrypsin PiMZ subjects. Thorax. 2006;61:129–133
  21. Yamamoto C, Yoneda T, Yoshikawa M, et al. Airway inflammation in COPD assessed by sputum levels of interleukin-8. Chest. 1997;112:505–510
  22. Jimenez LA, Drost EM, Gilmour PS, et al. PM10-exposed macrophages stimulate a proinflammatory response in lung epithelial cells via TNF-alpha. Am J Physiol Lung Cell Mol Physiol. 2002;282:L237–L248
  23. Mudway IS, Stenfors N, Duggan ST, et al. An in vitro and in vivo investigation of the effects of diesel exhaust on human airway lining fluid antioxidants. Arch Biochem Biophys. 2004;423:200–212
  24. Rogan MP, Geraghty P, Greene CM, et al. Antimicrobial proteins and polypeptides in pulmonary innate defence. Respir Res. 2006;7:29
  25. Cooke MS, Lunec J, Evans MD. Progress in the analysis of urinary oxidative DNA damage. Free Radic Biol Med. 2002;33:1601–1614
  26. Rozalski R, Winkler P, Gackowski D, et al. High concentrations of excised oxidative DNA lesions in human cerebrospinal fluid. Clin Chem. 2003;49:1218–1221
  27. Griffiths HR, Moller L, Bartosz G, et al. Biomarkers. Mol Aspects Med. 2002;23:101–208
  28. Loft S, Svoboda P, Kasai H, et al. Prospective study of 8-oxo-7,8-dihydro-2′-deoxyguanosine excretion and the risk of lung cancer. Carcinogenesis. 2006;27:1245–1250

PII: S0954-6111(07)00230-2

doi: 10.1016/j.rmed.2007.05.018

Respiratory Medicine
Volume 101, Issue 10 , Pages 2108-2112 , October 2007

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